By ACI Committee 555
This record offers info on elimination and reuse of hardened concrete. counsel for review of concrete constructions for entire or partial demolition is equipped. The applicability, benefits, barriers, and security concerns of varied varieties of concrete elimination tools, together with hand instruments, hand-operated energy instruments, vehicle-mounted gear, explosive blasting, drills and saws, nonexplosive demolition brokers, mechanical splitters, heating and thermal instruments, and hydrodemolition (water-jet blasting), are supplied. The to be had floor removing systems,their possible functions, and merits and drawbacks of varied sorts of floor elimination platforms are mentioned. issues for comparing and processing waste concrete for creation of aggregates compatible for reuses in concrete development are awarded.
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Extra resources for ACI 555R-01: Removal and Reuse of Hardened Concrete
9. It is important to employ a rotation or shaking speed that allows for good mixing of the contents of the tube. 10. The dilution factors for monoclonal antibodies are as a general rule 1/10, and 1/50 for CBMs, depending on the producer. 11. These monoclonal antibodies are specific for the HG of the outer cell wall layer and the fluorescent regions on the cell surface allow for easier recognition of cell cycle events and quantitative assessment of growth. 12. Nonfluorescent or dark zones between labeled regions of the wall represent newly formed cell wall (also see ref.
Notes 1. Agarose is required to prepare solid PGM. When making solid media 1–2 % of low melting agarose is added to PGM containing 20 % sucrose and incubated at 65 °C water bath until all the agarose is completely dissolved. Add the H3BO3 after dissolving the agarose. Then, it is poured into small Petri dishes or onto microscope slides and quickly stored in a wet chamber to avoid drying. Once the PGM solidifies take the flowers and gently brush them across the surface of the PGM to transfer the pollen grains.
01 g/L. 15. 18 g/L. 16. 006 g/L. 17. 00 g/L. 2 Chemical Treatment and Microscopy 1. 7- to 14-day-old Penium margaritaceum culture. 2. Chemicals to be screened. 3. MWC solution. 4. DMSO as control (see Note 3). 5. Carnation Instant nonfat milk. 6. JIM5 or LM18 monoclonal primary antibodies (Plant Probes, Leeds, UK) (see Note 4). 7. Fluorescein isothiocyanate (FITC)-Goat Anti-Rat secondary antibody (Invitrogen, #62-9511). 8. LSM 700 confocal laser scanning microscope (Carl Zeiss, Germany), Olympus Fluoview 1200 (Olympus, Center Valley, PA, USA), or other high resolution confocal microscope.