Download Cellular Fatty Acid-Binding Proteins II: Proceedings of the by Jan F. C. Glatz, Ger J. van der Vusse (auth.), Jan F. C. PDF

By Jan F. C. Glatz, Ger J. van der Vusse (auth.), Jan F. C. Glatz, Ger J. van der Vusse (eds.)

Two decades have elapsed for the reason that cytoplasmic proteins showing high-affinity binding of long-chain fatty acids have been first pointed out (Ockner et al., Science 177:56-58, 1972). those mobile fatty acid-binding proteins (FABPs) are actually good validated to include a ligand-defined crew of macromolecules belonging to a kinfolk of cytoplasmic lipid binding proteins. designated positive factors of the FABPs are the lifestyles of precise kinds of FABP and that those are present in quite a few tissues in outstanding abundance, with a few cells expressing a couple of style. The physiological value of the FABPs has in simple terms partially been elucidated. by way of expanding the cytoplasmic solubilization of fatty acids, the mobile FABPs are thought of to operate essentially in intracellular fatty acid delivery, yet can also be assigned very important regulatory roles in mobile lipid homeostasis in addition to within the modulation of cellphone progress and differentiation.
The wide pursuits in mobile FABPs has ended in the association of the first overseas Workshop on Fatty Acid-Binding Protein, held in Maastricht, the Netherlands, in 1989. triggered through the good fortune of the 1st assembly, the second foreign Workshop on Fatty-Acid-Binding Proteins, which used to be held back in Maastricht, on August 31 and September 1, 1992, introduced jointly clinical scpecialists within the box of FABP study for 2 days of in depth and fruitful dialogue. This quantity is a set of chosen papers from this convention, and hence presents the cutting-edge wisdom of mobile FABPs. The individuals to this factor symbolize pioneering in addition to new investigators, and likewise mirror the multidisciplinary nature of analysis during this intriguing and swiftly progressing box.

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Additional info for Cellular Fatty Acid-Binding Proteins II: Proceedings of the 2nd International Workshop on Fatty Acid-Binding Proteins, Maastricht, August 31 and September 1, 1992

Example text

Since many lipid-binding proteins exhibit no enzymatic activity, binding parameters provide an important quantitative measure for comparing the 'activity' of various wild-type and mutant forms. For this purpose, binding assays that are quantitative, accurate and robust are desirable. For the intracellular fatty acid- and lipid-binding proteins, a variety of biochemical and biophysical binding assays have been used. The biochemical assays include those based on gel-filtration [1-3], equilibrium dialysis [4], Lipidex ' [5,6] and liposomes [7].

However, other lipids exist in 'melted' phases such as bilayers, micelles or monomeric dispersions under these conditions. These lipids include unsaturated fatty acids, bile salts, lysophospho-lipids, and acyl CoA's. Although the lipid may not be in a monomeric state in the syringe, equilibrium between the micellar- or bilayer-phase lipid and the protein is rapid upon mixing. As illustrated above for oleate, which existed in a bilayer 36 GLYCO-CHENODEOXYCHOLATEfILBP GL YCO-CHOLATEfILBP 2000 4000 0000 0000 10000 2000 ·20 I ~ eooo 6000 10000 Time (sec) Time (sec) Ilcal «XlO ·30 ·60 ·90 j I Injection Number Injection Number "- '--l~o NH OH OH ~O OH Fig.

The concentrations of the probe in each phase were again measured and the concentration of unbound ll-DAP-[ll3H]undecanoate in the aqueous phase for each fatty acid: BSA ratio was determined from the concentration of probe in the corresponding heptane phase and its partition coefficient. Miscellaneous Protein was quantitated by the method of Lowry et al. [19] using BSA as the standard. 005% bromophenol blue. SDS-PAGE was performed as described by Laemmli [20]. Fluorography was performed as described by Bonner and Laskey [21].

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